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KMID : 0379119990270060399
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Korean Journal of Mycology
1999 Volume.27 No. 6 p.399 ~ p.405
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Intergeneric Hybrid Constructed by Nuclear Transfer of Saccharomycopsis into Saccharomyces
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Abstract
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Intergeneric hybrids between Saccharomyccopsis fiburigera KCTC 7393 and Saccharomyces cerevisiae KCTC 7049 (tyr-, ura-) were obtained by nuclear transfer technique. Nuclei isolated from the wild type S. fiburigera strain were transfered into auxotrophic S. cerevisiae mutants and new strains showing an increased starch degrading capability were selected. Maximum production of protoplasts was obtained from the treatment with 0.1% Novozym 234 at 30℃ for 90 min, and most effective osmotic stabilizer for the isolation of protoplasts was 0.6 M KCl at pH 5.8. The frequency of protoplast regeneration was 14.64% under the conditions. Genectic stability, conidial size, DNA content, and nuclear stain suggested that the fusants were aneuploidy. The specific activity of α-amylase was observed to increase about 1.2∼1.9 folds.
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